The Immunoassay Handbook: Theory and Applications of Ligand Binding PDF Free Download



The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research.

Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation.

The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip.

This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry.

Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing. is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book.

David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing.

The Immunoassay Handbook. Theory and Applications of Ligand Binding, ELISA and Related Techniques Pdf Features


Front Matter, Page iii
Copyright, Page iv
Foreword, Pages ix-x, Rosalyn Yalow
Preface, Pages xi-xiii, David Wild
Acknowledgements, Page xv, David Wild
List of Contributors, Pages xvii-xix, Marcos Alcocer, Zane D. Amenhotep, Philip R. Andersen, David Armbruster, John Backus, Steven R. Binder, Ronald R. Bowsher, Catherine A. Brennan, Carey-Ann D. Burnham, Daniel W. Chan, Richard S. Chapman, Nic D. Christofides, Penny M. Clark, Alastair H. Dent, John L. Dessi, Sushil G. Devare, Christopher Doern, Gordon Duffin, David C. Duffy, Sherry A. Dunbar, et al.
About the Editors, Page xxi
Chapter 1.1 – How to Use This Book, Pages 3-5, David Wild
Chapter 1.2 – Immunoassay for Beginners, Pages 7-10, David Wild
Chapter 1.3 – Immunoassay Performance Measures, Pages 11-26, Chris Davies
Chapter 2.1 – Principles of Competitive and Immunometric Assays (Including ELISA)1, Pages 29-59, Chris Davies
Chapter 2.2 – Non-competitive Immunoassays for Small Molecules—the Anti-complex and Selective Antibody Systems, Pages 61-65, Colin H. Self, Stephen Thompson, Theresa Street, Kelly J. Lamb, Gordon Duffin, John L. Dessi, Maggie Turnbull
Chapter 2.3 – Homogeneous Immunoassays, Pages 67-87, Edwin F. Ullman
Chapter 2.4 – Lateral Flow Immunoassay Systems: Evolution from the Current State of the Art to the Next Generation of Highly Sensitive, Quantitative Rapid Assays, Pages 89-107, Brendan O’Farrell
Chapter 2.5 – Ambient Analyte Assay, Pages 109-121, Roger Ekins
Chapter 2.6 – Free Analyte Immunoassay, Pages 123-137, Nic D. Christofides
Chapter 2.7 – Qualitative Immunoassay—Features and Design, Pages 139-147, Jianwen He, Simon Parker
Chapter 2.8 – Detection of Antibodies Relevant to Infectious Disease, Pages 149-155, Steven Binder, Jennifer A. Isler
Chapter 2.9 – Microsphere-Based Multiplex Immunoassays: Development and Applications Using Luminex® xMAP® Technology, Pages 157-174, Sherry A. Dunbar, Michaela R. Hoffmeyer
Chapter 2.10 – Lab-on-a-Chip, Micro- and Nanoscale Immunoassay Systems, and Microarrays, Pages 175-202, Simon Rattle, Oliver Hofmann, Christopher P. Price, Larry J. Kricka, David Wild
Chapter 2.11 – Immunological Biosensors, Pages 203-207, Jason Reed, James K. Gimzewski
Chapter 2.12 – Surface Plasmon Resonance in Binding Site, Kinetic, and Concentration Analyses, Pages 209-221, Robert Karlsson
Chapter 2.13 – Measurement of Single Protein Molecules Using Digital ELISA, Pages 223-242, David M. Rissin, David H. Wilson, David C. Duffy
Chapter 3.1 – Antibodies, Pages 245-265, Eryl Liddell
Chapter 3.2 – Signal Generation and Detection Systems (Excluding Homogeneous Assays), Pages 267-285, Ian Weeks, Larry J. Kricka, David Wild
Chapter 3.3 – Separation Systems, Pages 287-299, David Wild, Wlad Kusnezow
Chapter 3.4 – Conjugation Methods, Pages 301-313, Alastair H. Dent
Chapter 3.5 – Standardization and Calibration, Pages 315-322, David Wild, Chris Sheehan
Chapter 3.6 – Calibration Curve Fitting, Pages 323-336, John Dunn, David Wild
Chapter 4.1 – The Foundations of Immunochemistry, Pages 339-356, Robert F. Ritchie
Chapter 4.2 – Immunohistochemistry and Immunocytochemistry, Pages 357-377, Simon Renshaw
Chapter 5.1 – Practical Guide to ELISA Development, Pages 381-393, Jianwen He
Chapter 5.2 – Method Evaluation—A Practical Guide, Pages 395-402, Chris Sheehan, Jianwen He, Mari Smith
Chapter 5.3 – Interferences in Immunoassay, Pages 403-416, Jason Y. Park, Larry J. Kricka
Chapter 5.4 – Immunoassay Development in the In Vitro Diagnostic Industry, Pages 417-423, Doug Brandt, Steve Figard
Chapter 6.1 – Sample Collection, Including Participant Preparation and Sample Handling, Pages 427-440, Colin Wilde, Dorothée Out, Sara Johnson, Douglas A. Granger
Chapter 6.2 – Quality Assurance, Pages 441-454, Catharine M. Sturgeon
Chapter 6.3 – Point-of-Care Testing, Pages 455-463, James H. Nichols
Chapter 6.4 – Choosing an Automated Immunoassay System, Pages 465-468, Lori J. Sokoll, Daniel W. Chan
Chapter 6.5 – Immunoassay Troubleshooting Guide, Pages 469-506, David Wild, Jianwen He
Chapter 7.1 – Introduction to Immunoassay Product Technology in Clinical Diagnostic Testing, Pages 509-515, David Wild, Chris Sheehan, Steve Binder
Chapter 7.2 – Market Trends, Pages 517-531, David Huckle
Chapter 7.3 – Lateral Flow and Consumer Diagnostics, Pages 533-536, Sarah Tiplady
Chapter 7.4 – Consumer Diagnostics—Clearblue Pregnancy and Fertility Testing, Pages 537-540, Sarah Tiplady
Chapter 7.5 – The Triage® System, Pages 541-544, Thomas I. Koshy, Kenneth F. Buechler
Chapter 7.6 – Stratus® CS Acute Care™ STAT Fluorometric Analyzer, Pages 545-547, Amy Sarli, W.N. McLellan
Chapter 7.7 – IMx®1, Pages 549-554, Kent Ford
Chapter 7.8 – AxSYM®, Pages 555-559, Theresa Donahoe
Chapter 7.9 – Abbott ARCHITECT® Family of Analyzers, Pages 561-566, Frank A. Quinn, David A. Armbruster
Chapter 7.10 – ADVIA Centaur® XP, Pages 567-570, Jean Metzar
Chapter 7.11 – IMMULITE® and IMMULITE 1000, Pages 571-574, Arthur L. Babson
Chapter 7.12 – IMMULITE® 2000 and IMMULITE 2000 XPi, Pages 575-578, Arthur L. Babson
Chapter 7.13 – VITROS® ECiQ Immunodiagnostic System, VITROS® 3600 Immunodiagnostic System, and VITROS® 5600 Integrated System, Pages 579-584, John W. Backus, David Wild
Chapter 7.14 – VITROS® Immuno-Rate and MicroTip™ Assays, Pages 585-588, John W. Backus, Susan J. Danielson, David A. Hilborn
Chapter 7.15 – Access 2, Pages 589-596, Katherine M. Leith
Chapter 7.16 – DxI 600 and DxI 800, Pages 597-601, Katherine M. Leith
Chapter 7.17 – Elecsys® Immunoassay Systems, Pages 603-607, Mary Beth Myers
Chapter 7.18 – PRISM™, Pages 609-615, Dinesh Shah, Jim Stewart
Chapter 7.19 – Phadia Laboratory Systems, Pages 617-619, Angela Gore, Gareth Evans, Mats Rilvën
Chapter 8.1 – Immunoassay Applications in Veterinary Diagnostics, Pages 623-645, Thomas P. O’Connor Jr., John Lawrence, Philip Andersen, Valerie Leathers, Erwin Workman
Chapter 8.2 – Ligand Binding Assays in Drug Development, Pages 647-657, Jeffrey M. Sailstad, Ronald R. Bowsher, Omar F. Laterza, William Nowatzke
Chapter 9.1 – Clinical Concepts, Pages 661-672, Chris Davies
Chapter 9.2 – Thyroid, Pages 673-693, Rhys John, Nic Christofides, Carole A. Spencer, David Wild
Chapter 9.3 – The Adrenal Cortex, Pages 695-703, Les Perry, Sami Medbak
Chapter 9.4 – Bone and Calcium Metabolism, Pages 705-720, William D. Fraser, Kay W. Colston, John C. Stevenson
Chapter 9.5 – Infertility, Pages 721-734, Michael J. Wheeler
Chapter 9.6 – In Vitro Fertilization and Embryo Transfer (IVF-ET), Pages 735-743, Richard Fleming, Sherry Faye
Chapter 9.7 – Hirsutism and Virilization in the Female, Pages 745-756, Michael J. Wheeler
Chapter 9.8 – Pregnancy, Pages 757-776, Kevin Spencer, Tim Chard
Chapter 9.9 – Growth and Growth Hormone Deficiency, Pages 777-781, Gill Rumsby, Peter Hindmarsh, Michael Preece, Jane Pringle
Chapter 9.10 – Diabetes Mellitus, Pages 783-794, Penny Clark, Tim McDonald
Chapter 9.11 – Hematology, Pages 795-815, Katy Evans, Zane Amenhotep, Derek Dawson, Harry Waters, John Ardern
Chapter 9.12 – Cardiac Markers, Pages 817-831, Deborah French, Alan H.B. Wu
Chapter 9.13 – Cancer Markers, Pages 833-856, Hoon H. Sunwoo, Mavanur R. Suresh
Chapter 9.14 – Allergy, Pages 857-867, Marcos Alcocer, Lars Yman
Chapter 9.15 – Autoimmune Disease, Pages 869-890, David F. Keren
Chapter 9.16 – Gastrointestinal Tract, Pages 891-900, Arne Røseth, Richard Chapman, Radra Ramachandran, Chris Sheehan
Chapter 9.17 – Hepatitis, Pages 901-911, Ravi Kaul
Chapter 9.18 – HIV-1 and HIV-2: Causative Agents of AIDS, Pages 913-917, Catherine A. Brennan, Sushil G. Devare
Chapter 9.19 – Viral Diseases1, Pages 919-927, Carey-Ann D. Burnham, Christopher Doern, Steven R. Binder
Chapter 9.20 – Bacterial Diseases1, Pages 929-938, Carey-Ann D. Burnham, Christopher Doern, Steven R. Binder
Chapter 9.21 – Parasites and Fungi, Pages 939-943, Steven R. Binder, Patrice D. Sarfati
Chapter 9.22 – Therapeutic Drug Monitoring (TDM), Pages 945-962, Philip A. Routledge, Alun D. Hutchings
Chapter 9.23 – Drugs of Abuse, Pages 963-987, Alun D. Hutchings, Brian Widdop
Index, Pages 989-1013

Publisher‏:‎Elsevier Science; 4th edition (March 13, 2013)
Hardcover‏:‎1036 pages

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